Phytosanitary diagnostic, on-site
and epidemiology tools for Erwinia
Oeiras (Portugal), October 3th-4th, 2012
In the frame of WP2the Spanish partners of PhytFireled aphytosanitary laboratory training to which other groups contributed with inputs on the different methods to be disseminated among the various
parnters.Following quantitative and qualitative diagnostic techniques for E. amylovora, E. pyrifoliae and E. piriflorinigrans were presented:
Processing of samples of bacterial suspensions and of
plant tissue (EPPO, 2004);
DNA extraction for E. amylovora and E. pyrifoliae (Taylor et al., 2001);
DNA extraction for E.
piriflorinigrans (DNeasy Plant mini Kit – QIAGEN);
Real time PCRs for E.
amylovora (Pirc et al., 2009; Gottsberger et al., 2010);
Conventional PCR for E.
amylovora (Taylor et al., 2001);
LAMP for E. amylovora (Moradi et al., 2012);
Blue LAMP for E. amylovora (K. Stich and H. Halbwirth);
Conventional PCRs for E. pyrifoliae (Wensing et al., 2011; Kim et al., 2001) ;
Real time PCR E. pyrifoliae (based on Wensing et al., 2011);
Conventional PCR for E. piriflorinigrans (Barbé et al., in preparation);
Real time PCR for E.
piriflorinigrans (Barbé et al., in preparation);
Utilization of 'pregnancy test kit' for on-field
detection of E. amylovora
(Agristrip – Bioreba).